Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography.

نویسندگان

  • E Gimeno
  • A I Castellote
  • R M Lamuela-Raventós
  • M C de la Torre
  • M C López-Sabater
چکیده

A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (alpha-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol-water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%).

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عنوان ژورنال:
  • Journal of chromatography. A

دوره 881 1-2  شماره 

صفحات  -

تاریخ انتشار 2000